Genome Sequencing of Deep-Sea Fungus D. cejpii FS110

The strain D. cejpii FS110 (Accession No.KF706672) was isolated from a deep-sea sedimental sample in the South China Sea, and single colonies were incubated on a potato dextrose agar medium for five days at 30 °C [27 (link)]. The total genome DNA of D. cejpii FS110 was extracted using a Charge Switch gDNA Mini Bacteria Kit (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol. Genome DNA was checked using TBS-380 (Turner Biosystem Inc, Sunnyvale, CA, USA) for the production of high-quality dsDNA (OD260/280 = 1.8–2.0, >10 μg) and subsequent sequence. For the construction of a genome library, the purified genome DNA was broken into short fragments, which were used for Illumina sequencing (Illumina HiSeq Xten, San Diego, CA, USA) and Pacific Biosciences sequencing (Pacific Biosciences RS, San Diego, CA, USA), according to the manufacturer’s instructions. The fragments, including the Illumina and PacBio reads, were assembled by SOAP denovo v2.04 into many long scaffolds and finally integrated into a complete genome sequence [28 (link)]. This whole genome shotgun project of D. cejpii FS110 was deposited at DDBJ/ENA/GenBank under the accession SMSW00000000.