ELISAs (Quantikine kits; R&D Systems, Inc., Minneapolis, MN, USA) were used to quantify levels of uMMP-2, uMMP-9, uTIMP-1 and uTIMP-2 as previously reported (Smith et al, 2007 (link), 2008 (link)). Specimens, standards and reagents were prepared according to the manufacturer's instructions. Protein concentration in urine was determined by the Bradford method using bovine serum albumin as the standard as previously described by us (Moses et al, 1998 (link); Roy et al, 2004 (link); Pories et al, 2008 (link); Roy et al, 2008 (link)).
Protocol detail
Urinary Biomarker Quantification by ELISA
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Bovine serum albumin
Elisas
Corresponding Organization :
Other organizations : Boston Children's Hospital, Harvard University, Dana-Farber Cancer Institute
Top 5 similar protocols
Variable analysis
independent variables
- Not explicitly mentioned
- Levels of uMMP-2
- Levels of uMMP-9
- Levels of uTIMP-1
- Levels of uTIMP-2
- Protein concentration in urine
- Not explicitly mentioned
- Specimens, standards, and reagents were prepared according to the manufacturer's instructions
- Bovine serum albumin was used as the standard for protein concentration determination
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!