Calculating Fold Change Variability

P-values were calculated as described in the individual Fig legends. The average fold change (FC) in each experiment was calculated using the variable bootstrapping method, measuring the fold change between each potential pair of flies to determine the variability of the mean [12 (link)]. 95% confidence intervals (CI) were calculated using one sample t-test of log2FC values to determine the significance of distribution of the mean relative to the null using IBM SPSS Statistics Software 24 [60 ].

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Bhattacharya T., Newton I.L, & Hardy R.W. (2017). Wolbachia elevates host methyltransferase expression to block an RNA virus early during infection. PLoS Pathogens, 13(6), e1006427.

Publication 2017

SPSS Statistics Software 24

Flies

Corresponding Organization : Indiana University Bloomington

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