Modulating VMHc Neuron Activity
Corresponding Organization : Wuhan University
Other organizations : Fudan University, Shanghai Medical College of Fudan University, Peking University, University of Hong Kong
Variable analysis
- Treatment with 5 mM of MTZ (Sigma) in E3 water at 72 hpf for 4 h
- Incubation in 2 μM of SIS3 (Selleck), 25 μM of SB431542 (Tocris), or 20 μM of LY364947 (Selleck) from 76 to 124 hpf
- Not explicitly mentioned
- Incubation in 0.2% dimethyl sulfoxide (DMSO) (Thermo Fisher Scientific) from 76 to 124 hpf
- Positive control: Treatment with 5 mM of MTZ (Sigma) in E3 water at 72 hpf for 4 h
- Negative control: Incubation in 0.2% dimethyl sulfoxide (DMSO) (Thermo Fisher Scientific) from 76 to 124 hpf
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