Tg(vmhc:mCherry-NTR) larvae were treated with 5 mM of MTZ (Sigma) in E3 water at 72 hpf for 4 h as previously described (Zhang et al., 2013 (link)). After being washed, the larvae were then incubated in 2 μM of SIS3 (Selleck), 25 μM of SB431542 (Tocris), 20 μM of LY364947 (Selleck), or 0.2% dimethyl sulfoxide (DMSO) (Thermo Fisher Scientific) as control from 76 to 124 hpf.
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