Optimizing Cell Treatment Conditions

For cell treatment, OG (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in dimethyl sulfoxide (DMSO). For oral administration in mice, OG was dissolved in 40% PEG400 (Sigma-Aldrich) aqueous solution. Osteopontin (OPN; R&D Systems, Minneapolis, MN, USA) was dissolved in PBS to induce the EndoMT of 3B-11 cells (Supplementary Figure S1) [4 (link),5 (link)]. Recombinant HSP90α (rHSP90α) was purchased from Enzo Life Sciences Inc. (Farmingdale, NY, USA) and filtrated with 0.2-μm filters before use.

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Chua K.V., Fan C.S., Chen C.C., Chen L.L., Hsieh S.C, & Huang T.S. (2019). Octyl Gallate Induces Pancreatic Ductal Adenocarcinoma Cell Apoptosis and Suppresses Endothelial-Mesenchymal Transition-Promoted M2-Macrophages, HSP90α Secretion, and Tumor Growth. Cells, 9(1), 91.

Publication 2019

PEG400 Osteopontin (OPN;

Before Dimethyl sulfoxide Mice Oral administration Osteopontin Peg400

Corresponding Organization : National Chung Hsing University

Other organizations : National Taiwan University

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Variable analysis

independent variables

OG (Sigma-Aldrich, St. Louis, MO, USA)
Osteopontin (OPN; R&D Systems, Minneapolis, MN, USA)
Recombinant HSP90α (rHSP90α) from Enzo Life Sciences Inc. (Farmingdale, NY, USA)

dependent variables

EndoMT of 3B-11 cells

control variables

Dimethyl sulfoxide (DMSO)
40% PEG400 (Sigma-Aldrich) aqueous solution
Phosphate-buffered saline (PBS)

positive controls

None specified

negative controls

None specified

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