ON-TARGETplus SMARTpool control siRNA and MIF siRNA were purchased from Dharmacon (Lafayette, CO). The GBC cells were transfected with 10 nM of MIF siRNA or control siRNA using RNAiMAX (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions. Transfection was carried out as previously described [32 (link)]. Cells were subjected to invasion assay and viability assay 48 h post-transfection, unless otherwise stated.
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