CdrA-mediated Aggregation Assay

The CdrA-mediated aggregation assay was performed as previously described (12 (link)). d-Mannose, l-rhamnose, alginic acid, and sodium alginate from Sigma-Aldrich and uronic acids from Carbosynth LLC were used at 5 mg/mL. Bacterial alginate (Dextra Laboratories Ltd.) was used at 0.5 mg/mL due to poor solubility. Aggregation was visually evaluated and then measured by optical density readings taken at 600 nm. Percent relative aggregation was calculated by taking the difference between the OD₆₀₀ of the P_BAD-cdrAB strain and that of its corresponding vector control strain, dividing by the OD₆₀₀ of the vector control strain, and multiplying by 100%.