Quantitative Analysis of Total RNA

Total RNA was extracted from LNCaP cells with TRIzol reagent (Invitrogen, Carlsbad, CA, USA). The detection of the total RNA was performed using HiScript III RT SuperMix (Vazyme, Nanjing, China) and reverse transcription was carried out with HiScript III RT SuperMix (Vazyme, Nanjing, China). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was utilized as an internal standard. The primer sequences, as previously reported, are displayed in Table S2 (Wang et al., 2022 (link)).

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Zhang Q., Zhang P., Zhao Z., Wang J, & Zhang H. (2023). Exploring the role of differentially expressed metabolic genes and their mechanisms in bone metastatic prostate cancer. PeerJ, 11, e15013.

Publication 2023

TRIzol reagent HiScript III RT SuperMix

Glyceraldehyde 3 phosphate dehydrogenase Primer Reverse transcription Trizol

Corresponding Organization :

Other organizations : Taian City Central Hospital, Fourth People’s Hospital of Jinan, Qingdao Eighth People's Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total RNA expression

control variables

GAPDH used as an internal standard

controls

Positive control: None mentioned
Negative control: None mentioned

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