Characterization of Thyroid Cell Lines

The thyroid cell lines TPC1, Cal62, and 8505c were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). The sources of the cell lines have been described [57 (link)]. PCCl3-BRAF and PCCl3-HRAS were derived from PCCl3 rat thyroid follicular cells [58 (link)], to obtain doxycycline-inducible expression of BRAF^V600E or HRAS^V12 [57 (link), 59 (link), 60 (link)]. All cell lines used in this work were tested for mycoplasma contamination and authenticated every 6 months by short tandem repeat profiles using the Applied Biosystems Identifier kit in the Genomic Facility at the Institute of Biomedical Research (IIBm; Madrid, Spain).

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Ramírez-Moya J., Baker A.R., Slack F.J, & Santisteban P. (2020). ADAR1-mediated RNA editing is a novel oncogenic process in thyroid cancer and regulates miR-200 activity. Oncogene, 39(18), 3738-3753.

Publication 2020

Identifier kit

Braf Cell lines Doxycycline Eagle Fetal bovine serum Genomic Hras Mycoplasma Short tandem repeat Thyroid Thyroid follicular cells

Corresponding Organization :

Other organizations : Autonomous University of Madrid, Consejo Superior de Investigaciones Científicas, Instituto de Investigaciones Biomédicas Sols-Morreale, Beth Israel Deaconess Medical Center, Harvard University

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Variable analysis

independent variables

Doxycycline-inducible expression of BRAFV600E
Doxycycline-inducible expression of RASV12

dependent variables

Cellular characteristics and behavior of thyroid cell lines (TPC1, Cal62, and 8505c)

control variables

Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS)
Mycoplasma contamination testing and authentication of cell lines every 6 months

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