RNA was processed using the Ovation Complete Prokaryotic RNA-Seq Library System
(NuGEN, #0363–32, 0326–32, 0327–32) according to the manufacturer’s instructions
to a final pooled library concentration of 3 nM. Libraries were sequenced on an
Illumina HiSeq 2500 (SR50) at The Genomics Resource at the Fred Hutchinson
Cancer Research Center. Image analysis and base calling were performed using
Illumina's Real Time Analysis v1.18.66.3 software, followed by 'demultiplexing'
of indexed reads and generation of FASTQ files using Illumina's bcl2fastq
Conversion Software v1.8.4. Reads determined by the RTA software to pass
Illumina's default quality filters were concatenated for further analysis. The
FASTQ files were aligned and analyzed using Rockhopper software (McClure et al., 2013 (link)). These data have
been deposited to the GEO and are accessible using accession number
GSE150188.
(NuGEN, #0363–32, 0326–32, 0327–32) according to the manufacturer’s instructions
to a final pooled library concentration of 3 nM. Libraries were sequenced on an
Illumina HiSeq 2500 (SR50) at The Genomics Resource at the Fred Hutchinson
Cancer Research Center. Image analysis and base calling were performed using
Illumina's Real Time Analysis v1.18.66.3 software, followed by 'demultiplexing'
of indexed reads and generation of FASTQ files using Illumina's bcl2fastq
Conversion Software v1.8.4. Reads determined by the RTA software to pass
Illumina's default quality filters were concatenated for further analysis. The
FASTQ files were aligned and analyzed using Rockhopper software (McClure et al., 2013 (link)). These data have
been deposited to the GEO and are accessible using accession number
GSE150188.
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