GC-MS Analysis of Bacterial Extract

GC-MS analysis was performed as previously described17 (link). Analysis of MUSC 136^T extract was performed on an Agilent Technologies 6980N (GC) equipped with 5979 Mass Selective Detector (MS) using a HP-5MS (5% phenyl methyl siloxane) capillary column of dimensions 30.0 m × 250 μm × 0.25 μm and helium as carrier gas at 1 mL/min. The column temperature was programmed initially at 40 °C for 10 min, followed by an increase of 3 °C/min to 250 °C and was kept isothermally for 5 min. The MS was operating at 70 eV. The constituents were identified by comparison of their mass spectral data with those from NIST 05 Spectral Library.