Messenger RNA was isolated from frozen tumour material for gene expression analysis using the RNeasy Mini Kit (Qiagen, Venlo, the Netherlands). The Illumina HT-12 v4 chip was used to determine gene expression levels (Illumina, San Diego, CA, USA). Hypoxia- and angiogenesis-related factors were selected based on the literature regarding angiogenesis in UM (Table S4). Probes for CD3, CD8, and CD68 were previously validated [28 (link)].
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