IL-25 and IL-33 Stimulation of Effector CD4+ T Cells

Purified effector CD4⁺ T cells isolated from the lung of day 14–infected WT or Il17rb^−/− mice at 2 × 10⁵ cells were stimulated with recombinant IL-25 and/or IL-33 (R&D Systems) at 10 ng/mL in 96-well culture plate at 37 °C, 5% CO₂^{54 (link)–56 (link)}. After 36 h, the culture supernatant and stimulated cells were collected to analyze cytokine production via enzyme-linked immunosorbent assay (ELISA) and gene expression via RNA sequencing or real-time polymerase chain reaction (real-time PCR), respectively.

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Hansakon A., Jeerawattanawart S, & Angkasekwinai P. (2023). Differential and cooperative effects of IL-25 and IL-33 on T helper cells contribute to cryptococcal virulence and brain infection. Scientific Reports, 13, 9895.

Publication 2023

recombinant IL-25 IL-33

Cd4 t cells Cells Cytokine Enzyme linked immunosorbent assay Gene expression Il 25 Il 33 Lung Mice Real time pcr

Corresponding Organization :

Other organizations : Thammasat University

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Variable analysis

independent variables

Genotype (WT or Il17rb−/−)
Stimulation with recombinant IL-25 and/or IL-33

dependent variables

Cytokine production (measured by ELISA)
Gene expression (measured by RNA sequencing or real-time PCR)

control variables

Purified effector CD4+ T cells isolated from the lung of day 14–infected mice
Cell density (2 × 105 cells)
Cell culture conditions (37 °C, 5% CO2)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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