Transient expression of MdSYP121 and MdSNAP33 in tobacco

Full-length MdSYP121 and MdSNAP33 were fused to yellow fluorescent protein (YFP) vectors to pUC-SPYCE-35S and pUC-SPYNE-35S, respectively, by T4 recombination. The primers used are listed in Supplementary Table S1. These two recombinant plasmids were transiently expressed in tobacco leaves by A. tumefaciens (GV3101)-mediated infiltration^{28 (link)}. The YFP fluorescence of tobacco leaves was detected after infiltration for 4 days using a LSM 880 META confocal microscope (Carl Zeiss, Germany).

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He X., Huo Y., Liu X., Zhou Q., Feng S., Shen X., Li B., Wu S, & Chen X. (2018). Activation of disease resistance against Botryosphaeria dothidea by downregulating the expression of MdSYP121 in apple. Horticulture Research, 5, 24.

Publication 2018

LSM 880 META confocal microscope

Confocal microscope Fluorescence Plasmids Primers Protein Recombination Tobacco Vectors

Corresponding Organization : Shandong Agricultural University

Other organizations : Qingdao Agricultural University

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Variable analysis

independent variables

Transient expression of the recombinant plasmids pUC-SPYCE-35S::MdSYP121 and pUC-SPYNE-35S::MdSNAP33 in tobacco leaves

dependent variables

YFP fluorescence of tobacco leaves

control variables

Tobacco leaves used for expression
Infiltration of A. tumefaciens (GV3101) strain
Confocal microscope (LSM 880 META) used for detection

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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