Metabolite Identification in Human Urine

Glycine, N¹-acetylspermine,
GABA, N-(3-aminopropyl)pyrrolidin-2-one, acetyl chloride,
and sodium triacetoxyborohydride were obtained from Sigma Aldrich
(St. Louis, MO). LC–MS grade water, methanol, and acetonitrile
were from Honeywell Burdick & Jackson (Muskegon, MI). Formic acid
was obtained from Fischer Scientific (Pittsburgh, PA). Bulk human
urine was obtained from Gemini Bio-Products (West Sacramento, CA).
Fresh human urine was obtained from healthy donors at Colorado State
University. Patient urine for metabolite identification was from a
subset of the samples used for metabolite discovery in the previously
published study.^{10 (link)}

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Fitzgerald B.L., Mahapatra S., Farmer D.K., McNeil M.R., Casero RA J.r, & Belisle J.T. (2017). Elucidating the Structure of N1-Acetylisoputreanine: A Novel Polyamine Catabolite in Human Urine. ACS Omega, 2(7), 3921-3930.

Publication 2017

Glycine N1-acetylspermine acetyl chloride sodium triacetoxyborohydride LC–MS grade water methanol

Acetyl chloride Bulk Donors Gaba Glycine Human Methanol Patient Sodium Urine

Corresponding Organization :

Other organizations : Colorado State University, Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University

Top 5 similar protocols

Variable analysis

independent variables

Glycine
N1-acetylspermine
N-(3-aminopropyl)pyrrolidin-2-one
Acetyl chloride
Sodium triacetoxyborohydride

dependent variables

Metabolite identification

control variables

LC–MS grade water
Methanol
Acetonitrile
Formic acid
Bulk human urine
Fresh human urine
Patient urine

Annotations

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