Transcriptional Regulation in Adipose Tissue

Values are presented as means ± SEM and expressed relative to the respective control group. Group differences were assessed by Student’s t test, one-way analysis of variance (ANOVA), or two-way ANOVA where appropriate followed by Tukey’s post hoc test. Data were tested for normality before the use of a parametric test. Venn diagrams were created using the VennDiagram package (version 1.6.20) in R Studio. Gene overlaps presented in Fig. 1KESR1/Esr1 by gene correlations were calculated in adipose tissue from METSIM, MyoGlu, and HMDP studies using the midweight bicorrelation function in the weighted gene correlation network analysis package (version 1.67) in R Studio; significant correlations were set a priori as P < 0.001. Statistical significance of overlap for Venn diagrams was determined for each pairwise overlap using the hypergeometric probability formula. The represent ation factor (RF) indicates the fold change in observed versus expected overlap (R package version 1.24.0) (72 (link)). Statistical significance was established a priori at P < 0.05 for all other comparisons (GraphPad Prism 7.0).

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Zhou Z., Moore T.M., Drew B.G., Ribas V., Wanagat J., Civelek M., Segawa M., Wolf D.M., Norheim F., Seldin M.M., Strumwasser A.R., Whitney K.A., Lester E., Reddish B.R., Vergnes L., Reue K., Rajbhandari P., Tontonoz P., Lee J., Mahata S.K., Hewitt S.C., Shirihai O., Gastonbury C., Small K.S., Laakso M., Jensen J., Lee S., Drevon C.A., Korach K.S., Lusis A.J, & Hevener A.L. (2020). Estrogen receptor α controls metabolism in white and brown adipocytes by regulating Polg1 and mitochondrial remodeling. Science translational medicine, 12(555), eaax8096.

Publication 2020

VennDiagram GraphPad Prism 7.0

Adipose tissue Gene Gene function Hmdp Prism Student

Corresponding Organization : University of California, Los Angeles

Other organizations : Howard Hughes Medical Institute, University of California, San Diego, VA San Diego Healthcare System, National Institute of Environmental Health Sciences, Research Triangle Park Foundation, King's College London, University of Eastern Finland, Kuopio University Hospital, Norwegian School of Sport Sciences, University of Oslo

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Variable analysis

independent variables

Group (experimental condition)

dependent variables

Gene expression or other measured outcomes (not explicitly mentioned)

control variables

Normality of data was tested before using parametric tests
Positive and negative controls not explicitly mentioned

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