Protocol detail

Whole Cell Lysate Western Blot

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Whole cell lysates were made in Aqueous Lysis Buffer (50mM Tris pH 7.5, 150mM NaCl, 10mM EDTA pH8.0, 0.2% Sodium azide, 50mM NaF, 0.5%NP40) and resolved on a gradient gel as previously described(20 (link)). Primary antibodies used were E-cadherin, Vimentin, Zeb1, Snail, Slug, Sox9, GAPDH, CSF1, Galectin-3, MASP1/3, Fibronectin (BD Biosciences)

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Dongre A., Rashidian M., Eaton E.N., Reinhardt F., Thiru P., Zagorulya M., Nepal S., Banaz T., Martner A., Spranger S, & Weinberg R.A. (2020). Direct and Indirect Regulators of Epithelial-Mesenchymal Transition (EMT)-mediated Immunosuppression in Breast Carcinomas. Cancer discovery, 11(5), 1286-1305.

Publication 2020

E-cadherin Vimentin Snail GAPDH Galectin-3 Fibronectin

Antibodies Buffer Cell Csf1 E cadherin Edta Fibronectin Galectin 3 Gapdh Nacl Slug Snail Sodium azide Sox9 Tris Vimentin

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Variable analysis

independent variables

Whole cell lysates made in Aqueous Lysis Buffer (50mM Tris pH 7.5, 150mM NaCl, 10mM EDTA pH8.0, 0.2% Sodium azide, 50mM NaF, 0.5%NP40)

dependent variables

Expression levels of E-cadherin, Vimentin, Zeb1, Snail, Slug, Sox9, GAPDH, CSF1, Galectin-3, MASP1/3, Fibronectin

control variables

Resolved on a gradient gel as previously described(20 (link))

controls

No positive or negative controls were explicitly mentioned.

Annotations

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