Transwell Assay for Analyzing Cell Migration and Invasion

Transwell assay was performed as mentioned previously to analyze cell migration and invasion ability [43 (link)]. Transfected cells were seeded into Transwell upper-chamber with serum-free medium, while medium containing 10% FBS was placed into lower chamber (Corning). For Transwell invasion assay, the chamber should be precoated with a thin-layer Matrigel (BD Biosciences) before cells were seeded. Consequently, the upper cells of chambers were removed using a cotton buds, and the bottom surface cells of chambers were fixed, stained and photographed.

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Chen K., Wang Y., Dai X., Luo J., Hu S., Zhou Z., Shi J., Pan X., Cao T., Xia J., Li Y., Wang Z, & Ma J. (2024). FBXO31 is upregulated by METTL3 to promote pancreatic cancer progression via regulating SIRT2 ubiquitination and degradation. Cell Death & Disease, 15(1), 37.

Publication 2024

lower chamber Matrigel

Corresponding Organization :

Other organizations : Bengbu Medical College, First Affiliated Hospital of Bengbu Medical College

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Variable analysis

independent variables

Transfection of cells

dependent variables

Cell migration ability
Cell invasion ability

control variables

Serum-free medium in Transwell upper-chamber
Medium containing 10% FBS in lower chamber
Matrigel coating in Transwell invasion assay

controls

Positive control: None specified
Negative control: None specified

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