Histone modification and PolII ChIP was performed as previously described17 (link),62 (link), respectively. Immunoprecipitation was done with antibodies: anti-H3 (Abcam, #ab1791), anti-H3K27me3 (Millipore, #07-449), H2AK119ub (Cell Signaling Technology, #8240), anti-H3K36me3 (Abcam, #ab9050). PolII antibodies were as in64 (link). All ChIP experiments were followed by qPCR with indicated primer pairs (Supplementary TableĀ 2).
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