Cellular Assay Reagent Procurement

Fetal bovine serum (FBS) was purchased from R&D Systems (cat# S11550) and FB Essence (FBE) was purchased from VWR (cat# 10803–034). Human plasma was provided by Dr. Michael Pichichero. Bovine serum albumin (BSA) was purchased from Fisher Scientific (cat #BP1600-100) and fatty acid-free BSA (FAF-BSA) was from Sigma (cat#126575). Proteinase K was from Takara Bio (cat#ST3041). Actinomycin D was from Fisher Scientific (#NC9856244). Lysophosphatidic acid (#L7260), Lysophosphatidylcholine (#L1881), Propidium Iodide (#P4170), and Centrifugal Filter Units (#UFC501008, #UFC505008, # UFC510008) were from Sigma. The MTT Cell Growth Assay Kit was from Fisher Scientific (#CT01).

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Xu L., Bajorski P, & Poligone B. (2024). Lysophosphatidic acid down-regulates human RIPK4 mRNA in keratinocyte- derived cell lines. PLOS ONE, 19(4), e0287444.

Publication 2024

Fetal bovine serum (FBS) FB Essence Bovine serum albumin (BSA) Proteinase K Actinomycin D Lysophosphatidic acid Lysophosphatidylcholine Propidium Iodide

Corresponding Organization : Rochester Institute of Technology

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Variable analysis

independent variables

Fetal bovine serum (FBS) from R&D Systems
FB Essence (FBE) from VWR
Human plasma from Dr. Michael Pichichero
Bovine serum albumin (BSA) from Fisher Scientific
Fatty acid-free BSA (FAF-BSA) from Sigma
Proteinase K from Takara Bio
Actinomycin D from Fisher Scientific
Lysophosphatidic acid from Sigma
Lysophosphatidylcholine from Sigma
Propidium Iodide from Sigma
Centrifugal Filter Units from Sigma
MTT Cell Growth Assay Kit from Fisher Scientific

dependent variables

Measured outcomes

control variables

Variables kept constant to ensure valid results

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

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