Asthma Airway Resistance Measurement

Experimental mice (control group, asthma group, vandetanib group and dexamethasone group) were anesthetized by IP administration of 1% sodium pentobarbital (10 mg/kg), and tracheostomized as previously described (Shi et al., 2020 (link); Li et al., 2023 (link); Peng et al., 2023 (link)). The anesthetized mice were ventilated with a flexiVent system (SCIREQ, Montreal, PQ, Canada). Then the measurements of the resistance of the respiratory system (Rrs) were conducted. Aerosolized ACh at 3.125, 6.25, 12.5, 25, and 50 mg/mL concentration were gradually added and the dose-response curves of different experimental groups (control, asthma, dexamethasone and vandetanib) were charted. The Rrs results were collected and analyzed in Flexiware 8 software.

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Zeng X., Xue L., Li W., Zhao P., Chen W., Wang W, & Shen J. (2024). Vandetanib as a prospective anti-inflammatory and anti-contractile agent in asthma. Frontiers in Pharmacology, 15, 1345070.

Publication 2024

flexiVent system

Corresponding Organization :

Other organizations : Minzu University of China

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Variable analysis

independent variables

Groups: control group, asthma group, vandetanib group and dexamethasone group
Aerosolized ACh concentrations: 3.125, 6.25, 12.5, 25, and 50 mg/mL

dependent variables

Resistance of the respiratory system (Rrs)

control variables

Anesthesia: 1% sodium pentobarbital (10 mg/kg) administered IP
Tracheostomy procedure as previously described
Ventilation using a flexiVent system

controls

Positive control: Not specified
Negative control: Control group

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