To determine the effect of VAP on ADSCs, ADSCs were treated with VAP at different concentrations (0, 6, 12, 25, and 50 μg/mL). To figure out the roles of PI3K/Akt and HIF-1α signaling in ADSCs, ADSCs were divided into four groups as follows: Control, VAP, VAP+LY294002 and VAP + Perifosine groups. Control group: cells received no treatment; VAP group: cells were treated with 25 μg/mL VAP; VAP+LY294002 group: cells were pre-treated with 30 μM LY294002 (PI3K family specific inhibitor, 19–142, Sigma-Aldrich) [19 (link)] for 2 h, and then treated with 25 μg/mL VAP for an indicated time; VAP + Perifosine group: cells were pre-treated with 10 μM Perifosine (Akt-specific inhibitor, SC0227, Beyotime) for 2 h, and then treated with 25 μg/mL VAP for an indicated time.
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Elucidating the Role of PI3K/Akt and HIF-1α in VAP-Treated ADSCs
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Corresponding Organization : Nanjing University of Chinese Medicine
Other organizations : 81th Hospital of PLA
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Variable analysis
independent variables
- VAP concentration (0, 6, 12, 25, and 50 μg/mL)
- LY294002 (PI3K family specific inhibitor) pre-treatment (30 μM, 2 h)
- Perifosine (Akt-specific inhibitor) pre-treatment (10 μM, 2 h)
- Effect of VAP on ADSCs
- Control group (no treatment)
- Positive control: None specified
- Negative control: Control group (no treatment)
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