Schistosoma Detection by qPCR in CVL and Swabs

Detection of the Schistosoma-specific internal-transcribed-spacer-2 (ITS2) target by qPCR was performed at LUMC, as previously described (Supplementary Data) [18 (link), 29 (link)]. DNA extraction of 200 µL of CVL or cervical or vaginal swab fluid was done with QIAamp spin columns (QIAGEN Benelux, Venlo, the Netherlands) according to manufacturer’s guidelines. The qPCR output was reported in cycle threshold values (Ct values), and parasite DNA loads were categorized by the following prespecified values: high (Ct < 30), moderate (30 ≤ Ct < 35), low (35 ≤ Ct < 50), and negative (no amplification) [30 (link)].

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Sturt A.S., Webb E.L., Himschoot L., Phiri C.R., Mapani J., Mudenda M., Kjetland E.F., Mweene T., Levecke B., van Dam G.J., Corstjens P.L., Ayles H., Hayes R.J., van Lieshout L., Hansingo I., Francis S.C., Cools P, & Bustinduy A.L. (2021). Association of Female Genital Schistosomiasis With the Cervicovaginal Microbiota and Sexually Transmitted Infections in Zambian Women. Open Forum Infectious Diseases, 8(9), ofab438.

Publication 2021

QIAamp spin columns

Cervical Schistosoma Vaginal

Corresponding Organization : London School of Hygiene & Tropical Medicine

Other organizations : Ghent University, Zambart, University of KwaZulu-Natal, Oslo University Hospital, Leiden University Medical Center

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Variable analysis

independent variables

Detection of the Schistosoma-specific internal-transcribed-spacer-2 (ITS2) target by qPCR

dependent variables

Cycle threshold values (Ct values)
Parasite DNA loads categorized as high (Ct < 30), moderate (30 ≤ Ct < 35), low (35 ≤ Ct < 50), and negative (no amplification)

control variables

DNA extraction of 200 µL of CVL or cervical or vaginal swab fluid using QIAamp spin columns (QIAGEN Benelux, Venlo, the Netherlands) according to manufacturer's guidelines

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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