Synchronized populations of worms were grown on control (L4440), dld-1(RNAi) (LLC1.3), or 1:20 dld-1(RNAi). Each population was monitored for development at the L4 stage and days 1, 5, and 10 of adulthood. At the L4 stage, worms from each population were transferred onto plates containing 50 μM fluorodeoxyuridine to prevent the birth of progeny for analysis at adult days 1, 5, and 10. On the appropriate days, L4 larvae to day 10 adults were collected from the plates and washed in 4 to 5 mL S basal in a 50 mL conical tube (obtained from Children’s Hospital of Philadelphia). Flow cytometry analyses of animal length were performed using a BioSorter (Union Biometrica), which measures the relative axial length of an object by an axial light loss detector, where time of flight (TOF) indicates animal length (63 (link)). TOF measurements were collected for approximately 100–300 worms per sample, with 4 biological replicates tested per condition.
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