UBM was prepared as previously described (66 (link)). Porcine urinary bladders from market-weight animals were acquired from Tissue Source, LLC. Briefly, the tunica serosa, tunica muscularis externa, tunica submucosa, and tunica muscularis mucosa were mechanically removed. The luminal urothelial cells of the tunica mucosa were dissociated from the basement membrane by washing with deionized water. The remaining tissue consisted of basement membrane and subjacent lamina propria of the tunica mucosa and was decellularized by agitation in 0.1% peracetic acid with 4% ethanol for 2 hours at 300 rpm. The tissue was then extensively rinsed with PBS and sterile water. The UBM was then lyophilized and milled into particulate form using a Wiley Mill with a #60 mesh screen.
Protocol detail
Decellularization of Porcine Urinary Bladder
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Animals
Basement membrane
Bladders
Ethanol
Lamina propria
Muscularis
Muscularis mucosa
Peracetic acid
Porcine
Sterile
Tissue
Tunica externa
Tunica mucosa
Tunica serosa
Urothelial
Corresponding Organization : McGowan Institute for Regenerative Medicine
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Variable analysis
independent variables
- Preparation method of UBM (as previously described in reference 66)
- Not explicitly mentioned
- Porcine urinary bladders from market-weight animals
- Removal of tunica serosa, tunica muscularis externa, tunica submucosa, and tunica muscularis mucosa
- Dissociation of luminal urothelial cells from basement membrane by washing with deionized water
- Decellularization of remaining tissue (basement membrane and subjacent lamina propria of the tunica mucosa) by agitation in 0.1% peracetic acid with 4% ethanol for 2 hours at 300 rpm
- Extensive rinsing of tissue with PBS and sterile water
- Lyophilization and milling of UBM into particulate form using a Wiley Mill with a #60 mesh screen
- No positive or negative controls were explicitly mentioned in the provided information.
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