Quantifying Inflammatory Cytokines in Murine Lacrimal Glands
Corresponding Organization :
Other organizations : Smith-Kettlewell Eye Research Institute, Massachusetts Eye and Ear Infirmary, Harvard University, Tufts University
Variable analysis
- Age of mice (young vs. aged)
- Levels of IL1β in lacrimal gland lysates
- Levels of IL33 in lacrimal gland lysates
- Lacrimal gland samples were lysed in PBS
- Freeze-thaw cycles (-80°C for 10 minutes, 37°C water bath for 5 minutes)
- ELISA kits used to quantify IL1β and IL33 levels were from BioLegend, San Diego, CA, USA
- Not explicitly mentioned
- Not explicitly mentioned
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