The lacrimal glands harvested from young and aged mice were lysed in PBS by three complete freeze-thaw cycles (−80 °C for 10 minutes, 37 oC water bath for 5 minutes). Levels of IL1β and IL33 in the lacrimal gland lysates were quantified using commercially available ELISA kits (BioLegend, San Diego, CA, USA), as per the manufacturer’s instructions.
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