Before HTT, an initial blood sample was collected using an EDTA.K2 anticoagulant tube. A total of 8 ml of venous blood was taken from the patients’ cubital vein. The initial physiological indicators (SBP, DBP, HR, SaO2, and Tcore) were also measured. After HTT, post-exercise blood samples were collected immediately, and physiological data were measured after 5-min breaking. Twenty-four hours after HTT, blood samples for the recovery period were gathered. The feces were collected in the morning of two HTTs as pre-training and post-training samples. Clean cotton swabs were used to collect fresh fecal samples (5–10 g, no mix of urine, disinfectant, and sewage) into 15 ml sterile test tubes. Furthermore, the contents of organ injury biomarkers in plasma were detected, including alanine aminotransferase (ALT), alkaline phosphatase (AST), alkaline phosphatase (ALP), bilirubin, lactic dehydrogenase (LDH), alpha-hydroxybutyric dehydrogenase (α-HBDH), creatinine, urea nitrogen, cholinesterase, creatine kinase, prothrombin time (PT), activated partial prothrombin time (APTT), international normalized ratio (INR), Na+, K+, white blood cell (WBC), platelet (PLT), and hemoglobin.
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