Immortalized Human Prostate Cell Lines

Prostate stromal (BHPrS-1) and epithelial (NHPrE-1 and BHPrE-1) cells were gifts from Dr. Simon Hayward (NorthShore Research Institute, Evanston, IL). NHPrE-1 and BHPrE-1 lines were generated from primary cells by spontaneous immortalization, whereas BHPrS-1 were immortalized using human telomerase reverse transcriptase (hTERT)^{30 (link)}. THP-1 leukemia, LNCaP, and 22RV1 prostate cancer lines were purchased from ATCC. C4–2B^{31 (link)} cells were provided by Drs. Ruoxiang Wu and Leland Chung (Cedars-Sinai, Los Angeles, CA). NHPrE-1 and BHPrE-1 cells were cultured in Dulbeccòs Modified Eaglès Medium (DMEM):F12 in 1:1 ratio supplemented with 0.4% bovine pituitary extract, insulin-transferrin-selenium mix, epidermal growth factor (10ng/ml), antibiotic-antimycotic mix and 5% FBS (Thermo Fisher Scientific). THP-1, LNCaP, C4–2b and 22Rv1 cells were cultured in Roswell Park Memorial Institute-1640 (RPMI) medium containing 10% fetal bovine serum (FBS) and BHPrS-1 cells with 5% FBS.