P. berghei and P. yoelii blood stage parasites were propagated in female Swiss mice (6–8 weeks old, from Janvier Labs). We used wild type P. berghei (ANKA strain, clone 15cy1) and P. yoelii (17XNL strain, clone 1.1), and GFP-expressing PyGFP and PbGFP parasite lines, obtained after integration of a GFP expression cassette at the dispensable p230p locus.25 (link)
Anopheles stephensi mosquitoes were fed on P. berghei or P. yoelii-infected mice using standard methods,72 (link) and kept at 21°C and 24°C, respectively. P. berghei and P. yoelii sporozoites were collected from the salivary glands of infected mosquitoes 21–28 or 14–18 days post-feeding, respectively. P. berghei and P. yoelii sporozoite infections were performed in female C57BL/6 or BALB/c mice, respectively (6 weeks old, from Janvier Labs), by intravenous injection in a tail vein. HepG2 (ATCC HB-8065), HepG2/CD8138 (link) and Hepa1-6 cells (ATCC CRL-1830) were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% fetal calf serum and antibiotics (Life Technologies), as described.7 (link) HepG2 and HepG2/CD81 were cultured in culture dishes coated with rat tail collagen I (Becton-Dickinson).
Anopheles stephensi mosquitoes were fed on P. berghei or P. yoelii-infected mice using standard methods,72 (link) and kept at 21°C and 24°C, respectively. P. berghei and P. yoelii sporozoites were collected from the salivary glands of infected mosquitoes 21–28 or 14–18 days post-feeding, respectively. P. berghei and P. yoelii sporozoite infections were performed in female C57BL/6 or BALB/c mice, respectively (6 weeks old, from Janvier Labs), by intravenous injection in a tail vein. HepG2 (ATCC HB-8065), HepG2/CD8138 (link) and Hepa1-6 cells (ATCC CRL-1830) were cultured at 37°C under 5% CO2 in DMEM supplemented with 10% fetal calf serum and antibiotics (Life Technologies), as described.7 (link) HepG2 and HepG2/CD81 were cultured in culture dishes coated with rat tail collagen I (Becton-Dickinson).
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