RNA Isolation Using TRIzol Reagent

RNA was isolated as previously described [36 (link)] by using TRIzol protocol (TRI Reagent, Sigma^®, St. Louis, MO, USA, No. T9424), in which chloroform (Sigma^® No. C2432) served as denaturing agent for the cells and isopropanol (Sigma^® No. 650447) was the precipitating agent for RNA. The precipitate was diluted in DEPC water (Ambion^®,Austin, TX, USA, No. AM9920), which also contains 1% SUPERaseIn RNase Inhibitor (Thermo Fisher^®, Waltham, MA, USA, No. AM2694).

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Cordido A., Vizoso-Gonzalez M., Nuñez-Gonzalez L., Molares-Vila A., Chantada-Vazquez M.D., Bravo S.B, & Garcia-Gonzalez M.A. (2022). Quantitative Proteomic Study Unmasks Fibrinogen Pathway in Polycystic Liver Disease. Biomedicines, 10(2), 290.

Publication 2022

TRI Reagent DEPC water SUPERaseIn RNase Inhibitor

Austin Cells Chloroform Isopropanol Rnase Trizol

Corresponding Organization : Fundación Pública Galega de Medicina Xenómica

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Variable analysis

independent variables

TRIzol protocol (TRI Reagent, Sigma®, St. Louis, MO, USA, No. T9424)
Chloroform (Sigma® No. C2432) as denaturing agent for the cells
Isopropanol (Sigma® No. 650447) as precipitating agent for RNA

dependent variables

RNA isolated from cells

control variables

DEPC water (Ambion®,Austin, TX, USA, No. AM9920) containing 1% SUPERaseIn RNase Inhibitor (Thermo Fisher®, Waltham, MA, USA, No. AM2694)

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