Immunohistochemistry analysis was performed using the following primary antibodies: CYP11B2 (CYP11B2-41-17) [32 (link)], HSD3B1 (Abnova), HSD3B2 (KALKG619) [13 (link)], Cry1 (Abgent), and Cry2 (Abcam), as detailed in Table S2 .
Formalin-fixed paraffin-embedded tumor samples were cut into sequential 2-μm-thick sections, and deparaffinized and stained at the Pathology Department using a fully automated Ventana BenchMark ULTRA stainer (Ventana, Tucson, AZ, USA), according to the manufacturers’ instructions. Binding of peroxidase-coupled antibodies was detected using the ultraView Universal DAB Detection Kit as a substrate, and the sections were counterstained with hematoxylin.
Formalin-fixed paraffin-embedded tumor samples were cut into sequential 2-μm-thick sections, and deparaffinized and stained at the Pathology Department using a fully automated Ventana BenchMark ULTRA stainer (Ventana, Tucson, AZ, USA), according to the manufacturers’ instructions. Binding of peroxidase-coupled antibodies was detected using the ultraView Universal DAB Detection Kit as a substrate, and the sections were counterstained with hematoxylin.
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