Evaluating AC-5216 Effects on Diabetic Parameters

To evaluate effects of AC-5216 on levels of PG, INS, TC, and TG, which are important parameters in diabetic animal models30 (link)36 (link), the blood was collected and the levels of parameters above were determined followed by the final behavioral test as the previous study with minor adjustments36 (link). The single touch glucometer (OneTouch Ultra 2; LifeScan, High Wycombe, UK) was used to determine the levels of PG collected from the tail vein of rats. The blood sample was centrifuged (2000 g, 25 min) at 4 °C, and supernatants were collected. All samples were maintained at −80 °C until further use. The samples were assayed and quantified by Enzyme Immunoassay kits, respectively (INS (Millipore, USA), TC (Cell Biolabs, USA), and TG (Abcam, USA)). Six samples in each group were used to determine optical density (OD) values, at 450 nm in enzyme-linked immunosorbent assay (ELISA) plate reader and used for statistical analyzes.