Protein isolation and western blot analysis were performed as described previously [17 (link)]. Primary antibodies used were p53 (DO-7, Dako, CA, USA), p21 (c-19, Santa Cruz), ANXA A1, ANXA A2 (Becton Dickinson) and β-actin (Abcam), followed by mouse secondary conjugated antibody (Abcam). Protein abundance was quantified by image analysis using the Kodak image station 4000MM.
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