LRRK2 G2019S Transgenic Mouse Brain Lysis

G2019S TG mice were purchased from The Jackson Laboratory (strain B6; C3-Tg [PDGFB-LRRK2*G2019S] 340D jmo/J, stock number 016575) (Ramonet et al. 2011 (link)) and were housed in a specific pathogen-free facility at the Dankook University Animal Facility with the approval of the Dankook University Institutional Animal Care and Use Committee (DKU-16-035). G2019S mice and littermates were sacrificed by cervical dislocation. Brains were removed from the skull and lysed by ice-cold PBS with 1% Triton X-100 and 1Xprotease inhibitor cocktail (Calbiochem). Brain lysates were homogenized using a 17-gauge needle, incubated for 30 min at 4°C, and centrifuged at 4,000×g for 10 min at 4°C. Each supernatant was transferred to new tube and analyzed by western blotting.