Physiological Parameters of Transgenic Poplar

To examine the physiological parameters of NT poplar and PeHKT1;1 transgenic lines, the three-year-old soil-grown poplar plants were treated with 0.8% (w/v) NaCl at different time points (0, 0.5, 1, 1.5, 2, and 6 h). The CAT, SOD, and POD activities were measured by leaves according to Li et al. [29 (link)] using the corresponding assay kits: total protein assay kit (BCA method, A045-2), catalase assay kit (visible light, A007-1), total superoxide dismutase (T-SOD) assay kit (hydroxylamine method, A001-1), and peroxidase assay kit (A084-3), according to the manufacturer’s respective manuals (Jiancheng Bioengineering Inc., Nanjing, China). The experiments were repeated at least five times. Statistical analyses were performed using SPSS 21.0 software (SPSS Inc., Chicago, IL, USA). Data were compared using one-way analysis of variance (ANOVA) followed by Duncan’s test.

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Xu M., Chen C., Cai H, & Wu L. (2018). Overexpression of PeHKT1;1 Improves Salt Tolerance in Populus. Genes, 9(10), 475.

Publication 2018

total protein assay kit catalase assay kit total superoxide dismutase ( T-SOD) assay kit peroxidase assay kit

Assay Catalase Hydroxylamine Nacl Peroxidase Physiological Plants Poplar Protein Superoxide dismutase Transgenic Visible light

Corresponding Organization : Nanjing Forestry University

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Variable analysis

independent variables

NaCl treatment (0.8% w/v)

dependent variables

CAT activity
SOD activity
POD activity

control variables

Three-year-old soil-grown poplar plants
NT poplar and PeHKT1;1 transgenic lines

controls

No positive or negative controls were explicitly mentioned in the provided information.

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