Soft Agar Colony Formation Assay
Corresponding Organization :
Other organizations : Tsinghua University, Shenzhen University Health Science Center, Institute of Biophysics, Chinese Academy of Sciences, Zhuhai People's Hospital
Protocol cited in 1 other protocol
Variable analysis
- Concentration of HHT
- Number of colonies formed
- Cell line (A549 and H1975 cells)
- Cell density (1000 cells per plate)
- Culture medium (DMEM or RPMI 1640)
- Agarose concentration (0.3% low-melting-point agarose)
- Serum concentration (10% FBS)
- Bottom layer composition (0.6% agarose and 10% FBS)
- Incubation time (2 weeks)
- Staining method (0.5 mL of 0.005% crystal violet)
- Untreated cells as negative control
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