C57BL/6, B6.RAG1−/−Tyrp1B-w TRP-1 TCR transgenic, and B6.OPG−/− mice were purchased from The Jackson Laboratory. Mice were treated intraperitoneally with 100 µg anti-RANKL (IK22/5) or isotype control (2A3; Bio X Cell) antibody three times per week as stated in the text. B6.OT-II, B6.RIP-mOVA, and B6.IRBP−/− mice were described previously (Anderson et al., 2005 (link); DeVoss et al., 2006 (link)). Mice were treated at 3–5 wk of age and harvested at time points indicated in the text. All mice were housed and bred in specific pathogen-free conditions in animal facilities at UCSF or UNC, Chapel Hill. Animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at UCSF or UNC, Chapel Hill.
Protocol detail
Murine Immune Regulation Experiments
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Animal
Antibody
Cell
Institutional animal care and use committee
Isotype
Mice
Rag1
Rankl
Specific pathogen free
Transgenic
Trp 1
Corresponding Organization : University of California, San Francisco
Other organizations : University of North Carolina at Chapel Hill
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Variable analysis
independent variables
- Anti-RANKL (IK22/5) antibody treatment
- Isotype control (2A3) antibody treatment
- Not explicitly mentioned
- C57BL/6 mice
- B6.RAG1−/− mice
- Tyrp1B-w TRP-1 TCR transgenic mice
- B6.OPG−/− mice
- B6.OT-II mice
- B6.RIP-mOVA mice
- B6.IRBP−/− mice
- Mice were housed and bred in specific pathogen-free conditions
- Mice were treated at 3–5 wk of age and harvested at time points indicated in the text
- Isotype control (2A3) antibody treatment
- No explicit negative control is mentioned
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