Silibinin, trans-chalcone (benzylideneacetophenone), thioflavin S, and Congo red were purchased from Sigma (USA). Regular insulin was purchased from EXIR Pharmaceutical Company (Iran). Chloroform, monopotassium phosphate (KH2PO4), potassium phosphate (KH2PO), and dimethyl sulfoxide (DMSO) were purchased from Merck (Germany).
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