Enzymatic Assays for Biochemical Markers

D-lactic acid (D-LA) was determined by visible spectrophotometric method with kit provided by Shanghai Haling Biotechnology Co., Ltd. Superoxide dismutase (SOD) activity was measured by the xanthine oxidase method. The activities of alkaline phosphatase (AKP), acid phosphatase (ACP), diamine oxidase (DAO), and lysozyme (LZM) were assayed by p-nitrophenyl phosphate method, visible spectrophotometric method, spectrophotometric method, and turbidimetric method, respectively. The corresponding kits were provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

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Lin X., Zhang C., Cao K., Li Z., Zhao Z., Li X, & Leng X. (2023). Dietary Sodium Butyrate Changed Intestinal Histology and Microbiota of Rainbow Trout (Oncorhynchus mykiss), but Did Not Promote Growth and Nutrient Utilization. Aquaculture Nutrition, 2023, 3706109.

Publication 2023

Acid phosphatase Alkaline phosphatase D lactic acid Diamine oxidase Lysozyme Nitrophenyl phosphate Spectrophotometric Superoxide dismutase Turbidimetric Xanthine oxidase

Corresponding Organization : Shanghai Ocean University

Other organizations : Zhumadian Central Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

D-lactic acid (D-LA)
Superoxide dismutase (SOD) activity
Alkaline phosphatase (AKP) activity
Acid phosphatase (ACP) activity
Diamine oxidase (DAO) activity
Lysozyme (LZM) activity

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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