Eco-friendly Synthesis of Silver Nanoparticles Using Eucalyptus Leaves

Young Eucalyptus leaves from around 40 years old eucalyptus tree were collected from GT Road near Pindi Bypass, Gujranwala produce silver nanoparticles. Eucalyptus leaves were collected randomly, washed with tap water, and distilled water respectively. The small pieces of leaves were dried in shade to obtain the fine powder. Finally, the leaf powder was autoclaved under the pressure of 15Ib/sq inch and temperature of 121°C for 5 min [21 (link)]. Leaf powder of 5 g was boiled in 500 mL of distilled water (10 mg/mL) for 10 minutes and filtered by Whatman filter paper. The extract was stored at 4°C for further use. For silver nanoparticle synthesis, 2 mM silver nitrate solution was mixed with ELE (10 mg/mL) in different ratio. The change in color from yellow to dark brown represented the formation of AgNPs [21 (link)].