Metformin and Ethyl Acetate Fraction Effects on Diabetic Mice

A total of 508-week-old male C57BL6/J mice, including 40 db/db and 10 db/m mice, were purchased from Changzhou Cavens Laboratory Animal Co., Ltd [license key: SCXK (Su) 2016-0010, Jiangsu, China]. All experimental protocols in this research were approved by the Committee on the Ethics of Animal Experiments of the Jiangxi Normal University (Permission Number: JNU20210311-001). The breeding environment of mice was strictly controlled (25 ± 2°C, relative humidity 50 ± 5%, and 12/12 h diurnal cycle). After 1 week of adaptive feed, the 40 db/db mice were randomly divided into 5 groups: the diabetes group (Mod), the metformin group (250 mg/kg body weight per day, Met250), the low-dose group (100 mg/kg body weight per day, EAF100), the middle-dose group (250 mg/kg body weight per day, EAF250), and the high-dose group (400 mg/kg body weight per day, EAF400) and fed with D12451 during the period of drug administration. The non-diabetic mice (db/m) fed with D12450B only were used as the control group (Con). The drugs were dissolved in 5% Macrogol 400 solution; the Con and Mod groups were gavaged with a corresponding dose of 5% Macrogol 400 solution per day. The body weight and fast blood glucose (FBG) of all mice were measured weekly during the continuous administration for 8 weeks.