Lentiviral and HCV(JFH1) particle was propagated and tittered as described previously(20 (link)). Huh7 subgenomic replicon cells were established through G418(400ug/ml) selection upon in vitro transcribed subgenome transfection. HCV pseudo-particle system was propagated as previously described(21 (link)). Sendai virus(SeV) was obtained from Charles River Laboratories and the infection(10–100 HAU/ml) was conducted in serum-free DMEM for 1 hour at 37C.
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Propagation and Titration of Viral Particles
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Corresponding Organization : Southern California Reproductive Center
Other organizations : University of Nebraska Medical Center, University of British Columbia, Arkansas Children's Hospital, University of Arkansas for Medical Sciences, Northwest Nazarene University
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Variable analysis
independent variables
- Lentiviral particle propagation and titration
- HCV (JFH1) particle propagation and titration
- Huh7 subgenomic replicon cell establishment through G418 (400 μg/ml) selection upon in vitro transcribed subgenome transfection
- HCV pseudo-particle system propagation
- Sendai virus (SeV) infection (10–100 HAU/ml) in serum-free DMEM for 1 hour at 37°C
- Outcome of Lentiviral particle propagation and titration
- Outcome of HCV (JFH1) particle propagation and titration
- Outcome of Huh7 subgenomic replicon cell establishment
- Outcome of HCV pseudo-particle system propagation
- Outcome of Sendai virus (SeV) infection
- Protocol described previously (20 and 21)
- G418 concentration (400 μg/ml) for Huh7 subgenomic replicon cell selection
- Serum-free DMEM for Sendai virus (SeV) infection
- Infection duration (1 hour) for Sendai virus (SeV)
- Not explicitly mentioned
- Not explicitly mentioned
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