GC-MS Metabolome Profiling of Derivatized Samples

Lyophilized metabolome samples were derivatized using a solution of ethoxyamine hydrochloride in pyridine as the oximation reagent followed by silylation with N-trimethyl-N-trimethylsilylacetamide as described by [18 (link)]. GC-MS-analysis of the derivatized samples was performed using temperature gradient from 70°C to 320°C at a rate of 10°C/min on an Agilent 6890 N GC (Palo Alto, CA, USA) and an Agilent 5973 mass selective detector. 1 μl aliquots of the derivatized samples were injected in the splitless mode on a DB5-MS capillary column. Detection was performed using MS detection in electron impact mode (70 eV).