Isogenic human iPSC line WTC11 and GRN−/− iPSC line were generated by Dr. Bruce R. Conklin, as previously described (Miyaoka et al., 2014 (link)). GRN−/− iPSC and GRN−/− NGN2 iPSC were engineered (Figure S2F) and provided by Dr. Michael E. Ward (NIH) as previously described (Wang et al., 2017 (link)). iPSCs were cultured and maintained in Essential 8 Medium (Gibco, A1517001) on 6-well cell culture plates (Olympus, 25-105) coated with Vitronectin (Gibco, A14700) in DPBS. iPSCs were dissociated and passaged using EDTA (Invitrogen, AM9260G) in DPBS. This work was approved by UCSF GESCR Committee.
de Majo M., Koontz M., Marsan E., Salinas N., Ramsey A., Kuo Y.M., Seo K., Li H., Dräger N., Leng K., Gonzales S.L., Kurnellas M., Miyaoka Y., Klim J.R., Kampmann M., Ward M.E., Huang E.J, & Ullian E.M. (2023). Granulin loss of function in human mature brain organoids implicates astrocytes in TDP-43 pathology. Stem Cell Reports, 18(3), 706-719.
Corresponding Organization : University of California, San Francisco
Other organizations :
Institute for Neurodegenerative Disorders, Alector (United States), Tokyo Metropolitan Institute of Medical Science, Gladstone Institutes, Broad Institute, Harvard Stem Cell Institute, Harvard University, National Institute of Neurological Disorders and Stroke, National Institutes of Health
IPSCs were cultured and maintained in Essential 8 Medium on 6-well cell culture plates coated with Vitronectin in DPBS
IPSCs were dissociated and passaged using EDTA in DPBS
controls
Isogenic human iPSC line WTC11 (positive control)
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