Ultrasound-guided biopsies from SSAT and DSAT were performed in the paraumbilical region above and beneath the fascia Scarpa under visual control at the level of rectus abdominis muscle by needle suction technique after administration of local anesthesia (5–10 mL of 1% lidocaine, B. Braun, Melsungen, Germany) (24 (link)). Video loops were recorded to document the correct location at each biopsy.
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