Immunohistochemistry was performed as described previously [35 (link)]. Tumor sections prepared from control and silibinin-treated groups were stained with Ki67 (Thermo Fisher Scientific, Waltham, MA, USA) and c-Myc (clone 9E10; Santa Cruz Biotechnology, Dallas, Texas, USA), pSTAT3 (Cell Signaling Technology, Danvers, MA, USA) and GLUT1 (Abcam, Cambridge, UK) primary antibodies. Images were captured at 20X magnification using an inverted microscope (Leica, DMI600B) and processed by using Leica LAS AF software.
Free full text: Click here
