Cultivating A. muciniphila with Mucin and Glucose

A. muciniphila (ATCC-BAA-835) was purchased from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH and cultivated into liquid anaerobe basal medium (OXOID, ThermoFisher), supplemented with 0.05% porcine mucin type III (Sigma) and 4 g/L D-Glucose (Sigma) in an anaerobic workstation (Baker Ruskinn) at 37°C. The concentration of OSH, its precursors, and fibers to test was calculated based on the medium uptake ratio (MUR) of the OSH in these conditions, being that able of occupying half the volume of the culture tube when the hydrogel was fully hydrated in steady state (5 g/L). MUR was determined as described previously¹⁵ (link). All the experiments included a control tube with half the volume of the medium to avoid any concentration bias due to the absorption capacity of the OSH.