Human lung adenocarcinoma A549 and H1299 and human colorectal adenocarcinoma cells DLD1 were obtained from the Collection of Cell Lines of the Institute of Cytology RAS, Russia. Mouse colon carcinoma CT-26 cells were kindly provided by Prof. G. Multhoff (Technical University of München, Germany). A549, H1299 and DLD1 cells were cultivated in DMEM and CT-26 cells were grown in RPMI-1640 media supplemented with 10% heat inactivated fetal bovine serum (FBS) (HyClone, USA), 2 mM L-glutamine, 100 U/mL penicillin and 0.1 mg/mL streptomycin (PanEco, Russia) in a 5% CO2 atmosphere with 90% humidity. Viability was determined by 0.4% trypan blue exclusion.
The CT-26 luc and A549 luc cell lines were transduced with the luciferase gene using the pHIV-iRFP720-E2A-Luc [10 (link)] and pHIV-Luciferase vectors, respectively. The development of A549shHsp70 and DLD1shHsp70 cells was recently described [13 (link)].
The CT-26 luc and A549 luc cell lines were transduced with the luciferase gene using the pHIV-iRFP720-E2A-Luc [10 (link)] and pHIV-Luciferase vectors, respectively. The development of A549shHsp70 and DLD1shHsp70 cells was recently described [13 (link)].
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