Dharmacon ON-TARGETplus SMARTpool siRNA (20 nM) with DharmaFECT formulation #1 reagent (GE Life Sciences, Pittsburgh, PA) was used for Brd2, Brd3 and Brd4 knockdown [32 (link)]. INS-1 cells were seeded in a 24 well culture plate at 25,000 cells/well and, after 3 days, were transfected for 24 hrs. RNA was extracted 72 hrs after start of transfection. Knockdown was estimated from Affymetrix Gene Chip analysis.
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