The primary cancer group was induced by intraperitoneal diethylnitrosamine (Sigma-Aldrich, St. Louis, USA) injection in 15-day-old mice. The lack of matrillin-2 can increase the tumor proliferation in the liver [28 (link)]. The mice were investigated 4 months after the induction when liver tumors have reached the advanced multifocal status.
The metastatic group was induced by intrasplenic injection of a human melanoma HT168-M cell line (0.1 mL, ~ 5 × 105cells). This xenograft has high liver-colonizing capacity [29 (link)]. These mice were imaged only two months after the cell inoculation to mimic the early stage and the small size of metastatic lesions.
The metastatic group was induced by intrasplenic injection of a human melanoma HT168-M cell line (0.1 mL, ~ 5 × 105cells). This xenograft has high liver-colonizing capacity [29 (link)]. These mice were imaged only two months after the cell inoculation to mimic the early stage and the small size of metastatic lesions.
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